Measurement

Part:BBa_K3792004

Designed by: Caleb Molinari   Group: iGEM21_FSU   (2021-09-24)


BIOFAB apFAB98 Measurement Device

The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements.

Promoter Strength Measurement
Negative
Control
Negative Control1000
apFAB40
BBa_K3792003
apFAB40
BBa_K3792003
1700
apFAB98
BBa_K3792004
apFAB98
BBa_K3792004
1700
apFAB51
BBa_K3792005
apFAB51
BBa_K3792005
1700
molecules of equivalent PE-Texas Red (MEPTR)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
    Illegal AgeI site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None